Guidelines

Guidelines on the Performance of Surgery in Rodents

Unit for Laboratory Animal Medicine
Aug 25, 2023 12:00 am

This document is intended to provide guidelines for investigators who perform surgical procedure(s) in rodents such as mice, rats, hamsters, gerbils, guinea pigs, etc.

All surgical procedures, anesthetics, analgesics, antibiotics or other medications used on animals must be approved by the IACUC and described in the animal use protocol. Personnel performing the surgical procedure must be listed on the animal use protocol and appropriately trained in the technique prior to starting any surgeries.  

Any divergence from the recommendations in this document must be described in the animal use protocol prior to implementing the deviation.

  • Responsibility

    1. Principal Investigator and Surgeon: Responsible to ensure appropriate surgical preparation, technique and monitoring for each animal as well as to oversee the animal's post-operative recovery period.
  • Glossary Definitions

  • Procedures

    1. Documentation

    1. Rodent surgery and Post-Operative Records are required for every rodent that has undergone surgery.
    2. An individual rodent surgery and post-operative record must be used for each rodent cage that contains post-operative animals. Multiple animals from the same cage can be contained on the same rodent surgery and post-operative record, as long as the animals have unique identification, and the post-surgical record is completed in a way that allows for individual animal observations to be clearly identified. Each animal must have their own observations recorded daily.
      1. Daily post-operative recordings CANNOT be pre-recorded, i.e., each day’s recording can only be recorded at the time that the post-operative monitoring is occurring.
      2. Alternative post-operative records that include multiple cages on one record may be appropriate if individual animal tracking is still performed and only with approval of a faculty veterinarian.
    3. Rodent surgery and post-operative records must be contained within the animal room or within close proximity to the animals and readably retrievable for review by investigative, IACUC, or veterinary personnel at all times.
    4. Rodent surgery and post-operative records must be kept and maintained until sutures/wound clips are removed or the animal is euthanized.
      1. If the animal is euthanized or dies within the post-operative period, that must be noted on the post-operative record.
    5. Rodent surgery and post-operative records must remain near the animal for at least 7 days following surgery even if skin incisions are not closed with sutures or wound clips (i.e., surgical glue).
    6. Rodent surgery and post-operative records must include anesthetics and analgesics administered for the surgical procedure and after for post-operative care. The records must also include the frequency of monitoring by any laboratory and veterinary personnel during the post-operative period. Record the following information for both anesthetics and analgesics:
      1. Dose
      2. Frequency of administration
      3. Route of delivery
    7. Rodent surgery and post-operative records for mice and rats must be placed in the assigned folder within the animal room once the post-operative monitoring period is over. Records will be stored as outlined in the Guidelines on Medical Records for Investigative Personnel.
    8. Records for animals other than those of the genus Mus or Rattus bred specifically for research must be stored and maintained in accordance with the ULAM Guidelines for Animal Medical Records.

    2. Preparation of the Surgical Area

    1. According to the Guide for the Care and Use of Laboratory Animals: Eighth Edition, "For most survival surgery performed on rodents and other small species...the space should be dedicated to surgery and related activities when used for this purpose, and managed to minimize contamination from other activities conducted in the room at other times." (pg. 144)
      1. The surgical area should be a room or a portion of a room that is easily sanitized and not used for any other purpose during the time of surgery.
      2. Clean and disinfect the surface upon which the surgery will be performed with an approved environmental disinfectant before beginning the surgical procedure.

    3. Preparation of the Surgical Supplies

    1. Surgical instruments and supplies, e.g., suture material
      1. Use sealed, aseptic surgical supplies and supplies.
        1. Sterilization, such as steam sterilization (autoclaving), plasma vapor sterilization, ethylene oxide sterilization or vaporized hydrogen peroxide (for heat- or pressure-sensitive items) is required for all surgical instruments, supplies, and implants, unless used directly from sterile packaging from the supplier.
          1. If other methods of sterilization are required for research goals, they must be described and approved in your IACUC protocol.
        2. Refer to Guidelines for the Storage of Sterile Items
    2. Drapes
      1. While not required, drapes are highly recommended in order to maintain a sterile surgical field.
      2. If drapes are not being used, extra precautions must be taken in order to maintain appropriate aseptic technique. Consult with veterinary personnel to discuss the necessary extra precautions: ulam-vets@umich.edu or 734-936-1037. 
      3. Drapes can be cloth, paper, sterile stockinettes, 3M™ Steri-Drape™ Incise Drapes, or new and unused boxes of GLAD Press'n Seal® wrap.
        1. Sterilize drapes using an autoclave, vaporized hydrogen peroxide or ethylene oxide where appropriate (plastic products).
        2. Drapes can be used for wrapping instrument packs prior to sterilization as well as for creating a sterile field around the incision site.

    4. Preparation of the Animal

    1. Fasting is not required for rodents.
      1. Fasting longer than 6 hours must be scientifically justified in the approved IACUC protocol.
    2. Anesthesia
      1. Use an approved agent appropriate for the species AND the procedure.
        1. See Anesthesia and Analgesia Drug Descriptions for more information on anesthetic and analgesic options.
      2. Maintain the animal in a surgical plane of anesthesia throughout the duration of the procedure
        1. See Anesthesia and Sedation Monitoring Guidelines for more information on determining the surgical plane of anesthesia and monitoring during anesthesia.
    3. Skin Disinfection
      1. Remove hair from the surgical site prior to disinfecting the skin.
        1. Animal clippers, razors, depilatory cream (Nair®) or manual plucking can be used to remove hair from the surgical site.
        2. Ensure depilatory creams are:
          1. Not left on the animal’s body longer than appropriate to avoid irritation of the skin.
          2. Completely rinsed from the animal’s body before disinfecting the skin.
      2. Remove all visible debris from surgical site.
      3. Disinfect the surgical site with a recommended skin disinfectant to clean and remove bacteria from the surgical site and prevent the growth of disease-causing organisms.
        1. Perform a minimum of three alternating (inside to outside of prepped surgical area) scrubs of disinfectant (chlorhexidine scrub, iodine scrub, etc.) and rinse (warmed sterile saline, sterile water, or alcohol) after the removal of visible dirt and debris.
        2. 3M™ Avagard™ (Chlorhexidine Gluconate 1% Solution and Ethyl Alcohol 61% w/w) can be used in place of the triplicate application method of scrub and rinse described above.
          1. Dispense 1 pump of Avagard on sterile gauze.
          2. Working in a circular pattern, inside to outside of prepped area, apply Avagard.
          3. Let air dry (typically under 1 minute).
          4. Repeat steps 4.c.iii.3.a-c one more time.
      4. Place the drape around the surgical site after the skin has been appropriately disinfected, if using.

    5. Surgeon Preparation

    1. Wash hands thoroughly using soap and/or a disinfecting solution (e.g., chlorhexidine, iodine-based surgical scrubs, or 3M Avaguard hand antiseptic.)
    2. Required attire for the surgeon during the surgical procedure.
      1. Mask
      2. Sterile gloves
      3. Clean scrub top, disposable gown, or lab coat
        1. Follow vivarium PPE procedures when working within the vivarium, i.e., a gown is required and no lab coat.
    3. Wrap sterile gloves in individual pairs and can include:
      1. Commercially available sterile surgical gloves
      2. Commercially available sterile exam gloves
      3. Regular exam gloves, packaged and autoclaved with appropriate quality assurance measures to ensure sterility (autoclave tape, etc.).
    4. Don new sterile gloves if aseptic technique is disrupted for any reason (repositioning of the animal, touching anything outside of the sterile surgical field, etc.). Use of a non-sterile assistant can minimize the need to change sterile gloves within a procedure. 

    6. Performing Multiple Rodent Surgeries in Series

    1. Investigators should begin with at least 2 sets of sterile instruments.
      1. Maintain aseptic technique while cleaning gross debris from instruments followed by sterilizing with a hot bead sterilizer between each animal.
      2. Use one pack of instruments for up to 5 consecutive surgeries.
        1. Change to a new pack of sterile instruments sooner if unable to properly clean gross debris from instruments between animals.
    2. Use a new sterile suture piece for each animal.
    3. Use a new sterile drape for each animal.
    4. Use new sterile gloves for each animal.
    5. Disinfecting the surgical area between animals is recommended.

    7. Suture Materials & Wound Closure

    1. Surgery in which a body cavity such as the abdomen or thorax has been opened requires a two-layer closure to close the body wall separately from the skin.
    2. Select suture material (absorbable vs. non-absorbable; braided vs. monofilament) that is appropriate to the tissues, procedure, and activity of the animal.
    3. Remove wound clips and skin sutures 7-10 days after placement.

    8. Post-Operative Monitoring & Care

    1. Observe and monitor animals every 15 minutes during recovery from anesthesia until the animal is ambulatory.
      1. House rodents:
        1. In a clean, dry, enclosed space without risk of aspiration.
        2. individually until completely recovered to avoid cannibalism by cage mates.
      2. Continue monitoring parameters and thermal supplementation throughout the recovery period. See Anesthesia and Sedation Monitoring Guidelines for more information on post-operative monitoring and appropriate thermal support devices.
    2. Administer post-operative medications including analgesics, antibiotics and/or anesthetic reversals during the early recovery period and according to the approved protocol or the advice of a ULAM veterinarian.
    3. Place a yellow acetate with a Surgery Observation Sticker (SOS) over the cage card when the fully recovered animal is returned to the animal room. 
      1. Record on the SOS sticker:
        1. The date of surgery
        2. Circle the closure method used: skin glue, sutures, or wound clips
        3. The “report by” date, i.e., 10 days post-surgery
      2. Remove wound clips and skin sutures by 10 days after surgery unless described otherwise in an IACUC approved protocol or as recommended by a ULAM veterinarian to necessitate incomplete wound healing.
      3. Remove the yellow acetate with SOS sticker once the skin sutures or wound clips have been removed, or 7 days post-surgery if skin glue was used instead of sutures or wound clips.
    4. Investigative personnel must examine all post-surgical animals at least once a day for 7 – 10 days, or until the skin sutures or wound clips are removed.
      1. Daily post-operative monitoring and health status of the animals must be recorded during the post-operative monitoring period and records must be maintained in the rodent surgery and post-operative documentation (see Procedures section 1).

    9. Incision Repair

    1. Repair any incision site dehiscence following veterinary personnel consultation or in accordance with the IACUC approved protocol.
    2. Daily post-operative monitoring for 7-10 days from date of incision repair is required and records must be maintained in the rodent surgery and post-operative documentation (see Procedures section 1).

    10. Non-Survival Surgery

    1. Non-survival surgeries do not require aseptic techniques or dedicated facilities but should be performed in a clean area, free of clutter, and using acceptable veterinary sanitation practices equivalent to those used in a standard examination/treatment room. Personnel present in the area should observe reasonable cleanliness practices for both themselves and the animals."
  • Appendix A: Rodent Surgery and Post-Operative Record (Notebook Size)

  • Appendix B: Rodent Surgery and Post-Operative Record by Cage

  • Appendix C: Example of Rodent Surgery and Post-Operative Records

  • References

    1. Comparison of Aqueous and Alcohol-based Agents for Presurgical Skin Preparation Methods in Mice. Website at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6059221/
Species: Rats Mice
Topic: Surgery
Questions?

If you have questions or comments about this document, contact ULAM Veterinary Staff (ULAM-vets@umich.edu).

The ULAM Training Core (ULAM-trainingcore@umich.edu or 734-763-8039) can be contacted to provide training in techniques at no charge.

For any concerns regarding animal health after work hours or on holidays/weekends, contact DPS (3-1131) who will contact the on-call veterinarian.